SIG-M5

Cat.No.: CSC-C0495

Species: Human

Source: acute monocytic leukemia

Morphology: large round cells growing singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0495

Description

Established in 1995 from the bone marrow of a 63-year-old man with acute myeloid leukemia of monocytic origin (AML FAB M5a) at diagnosis

Species

Human

Source

acute monocytic leukemia

Recommended Medium

90% SuperCult® IMDM + 10% h.i. FBS.

Culture Properties

suspension

Morphology

large round cells growing singly in suspension

Karyotype

Human hypertriploid/hypotetraploid karyotype with 4% polyploidy - 78(77-81)<3n>XX/XXY, +1, +2, +3, +4, +5, +8, -9, -10, +12, +15, +16, +17, +18, +21, +22, +0-2mar - i(1q), add(17)(p1?1) - resembles published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 (+), CD14 (+), CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between FISH and ISH?

FISH and ISH both use the same concept of in situ hybridization, but FISH does so with the addition of fluorescent probes. This enables direct detection. Fluorescence allows the visualization of probes in combination with the surrounding cells and tissues.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good results

We purchased tumor cell products to study the molecular mechanism of lymphoma progression and drug resistance. Luckily, we obtained good experimental data.

14 Jan 2023

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