SHI-1

Cat.No.: CSC-C0630

Species: Human

Source: acute myelocytic leukemia

Morphology: round cells growing singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0630

Description

Established in 2002 from the bone marrow of a 37-year-old male with relapsed acute monocytic leukemia (AML-M5b); described to carry t(6;11)(q27;q23) with MLL-MLLT4 (MLL-AF6) rearrangement and a TP53 point mutation

Species

Human

Source

acute myelocytic leukemia

Recommended Medium

80% SuperCult® IMDM + 20% h.i. FBS.

Culture Properties

suspension

Morphology

round cells growing singly in suspension

Karyotype

Human pseudodiploid karyotype with 12% polyploidy; 46(44-46)<2n>XY, -18, +mar, t(6;11)(q27;q23), add(8)(p23), del(10)(p11), del(17)(p12); carries t(6;11) effecting rearrangement of MLL with MLLT4 (AF6); matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, CD41 +, CD235a -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 20 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is DMEM media in cell culture?

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Additional services

This company offers a range of additional services, including cell line authentication and mycoplasma testing, to ensure the integrity of research results.

03 Feb 2023

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