SH-2

Cat.No.: CSC-C0628

Species: Human

Source: acute myeloid leukemia

Morphology: round cells growing singly and partially in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0628

Description

Established in 2005 from the bone marrow of a 35-year-old Chinese man with AML-M2 after idarubicin/cysteine arabinoside treatment; cells were described to express natural killer cell-related antigens in addition to myeloid ones and to exhibit TP53 gene alteration; cells are tumorigenic in nude and SCID mice

Species

Human

Source

acute myeloid leukemia

Recommended Medium

90% SuperCult® IMDM + 10% h.i. FBS.

Culture Properties

suspension

Morphology

round cells growing singly and partially in clumps in suspension

Karyotype

Human hyperdiploid karyotype with 8% polyploidy; 57(54-58)<2n>X, -Y, +1, +2, +3, +4, +6, -11, +12, +13, +15, +18, +19, +22, +2mar, del(1)(q31.2q42)x2, der(4)t(1;4)(p22;q32), der(16)t(16;17)(q24;q12); related to published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD56 +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 12 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is in cell media?

Cell culture media generally comprise an appropriate source of energy and compounds which regulate the cell cycle. A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors.

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