SF-158

Cat.No.: CSC-C6199X

Species: Insect

Morphology: polymorphic round cells, mostly adherent, a few cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C6199X

Description

Cloned from the parent line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors

Species

Insect

Recommended Medium

80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace′s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate)

Culture Properties

suspension

Morphology

polymorphic round cells, mostly adherent, a few cells in suspension

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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LAT

Description: Etablished from the aortic tissue cells of a lamb; cells were described to be susceptible to Visna Maedi Virus and to be free of bovine viral diarrhoea virus (BVDV)

Cat#: CSC-C2760 INQUIRY

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SF-21

Description: Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells

Cat#: CSC-C6196X INQUIRY

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SCHNEIDER-2

Description: Established from the late embryo of a Drosophila melanogaster (fruitfly) in 1972; originally the cells were diploid with 5-10% XY, currently the cell line has only XX cells that are now 60-80% tetraploid; also often termed (incorrectly) S2

Cat#: CSC-C6197X INQUIRY

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