SD-1

Cat.No.: CSC-C1386

Species: Human

Source: peripheral blood B lymphoblastoid cells

Morphology: ovoid to round cells growing in suspension, singly or in clumps

Culture Properties: suspension

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Cat.No.

CSC-C1386

Description

Established from the peripheral blood of a woman with acute lymphoblastic leukemia (pre B-ALL) at diagnosis; cells were immortalized with EBV; shown at Creative Bioarray by RT-PCR to carry minor breakpoint BCR-ABL1 fusion for which it is a suitable positive control for RT-PCR

Species

Human

Source

peripheral blood B lymphoblastoid cells

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

ovoid to round cells growing in suspension, singly or in clumps

Karyotype

Human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9;22)(q34;q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transc

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are tumor cell markers?

A tumor marker is anything present in or produced by cancer cells or other cells of the body in response to cancer or certain benign (noncancerous) conditions that provide information about cancer.

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