RPMI-8402

Cat.No.: CSC-C0376

Species: Human

Source: T cell acute lymphoblastic leukemia

Morphology: round single cells in suspenson, growing partly in clumps

Culture Properties: suspenson

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Cat.No.

CSC-C0376

Description

Established from the peripheral blood of a 16-year-old woman with acute lymphoblastic leukemia (ALL) in 1972

Species

Human

Source

T cell acute lymphoblastic leukemia

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspenson

Morphology

round single cells in suspenson, growing partly in clumps

Karyotype

Human hypotetraploid karyotype with 2% polyploidy - 90(79-91)<4n>XXX, -X, +3, +3, -10, -13, -14, +15, -18, -20, +2mar, dup(4)(q13q23)x2, del(6)(q14q22)x2, t(11;14)(p15;q11)x2, add(15)(p13) - sideline with der(1)t(1;9)(p35/36;q11), add(13)(q34) - carries t

Quality Control

Mycoplasma: contaminaton was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, CD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRg

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between DMEM and MEM?

The major differences between DMEM and MEM include the use of lower concentrations of amino acids, vitamins, and inorganic phosphate and the inclusion of lactate and glutamine in the medium.

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