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Cat.No.: CSC-C6239X

Species: Human

Source: B lymphoblastoid cells

Morphology: round cells growing in suspension, singly or in small clusters

Culture Properties: suspension

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Cat.No.

CSC-C6239X

Description

Established from the peripheral blood of a 3-year-old boy with severe combined immunodeficiency (SCID) by EBV-transformation using the cell line B95-8 as source for EBV; cells were described as lacking MHC class II molecules

Species

Human

Source

B lymphoblastoid cells

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing in suspension, singly or in small clusters

Karyotype

Human near-diploid karyotype with 8% polploidy - 46(43-46)<2n>XY, no consistent abnormality present

Quality Control

Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR -, sm/cyIgG (5-10%)+, sm/cyIgM (20-40%)+, sm/cykappa (5-10%)+, sm/cylambda

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the classification of lymphomas?

Lymphomas are divided into two categories, Hodgkin lymphoma and non-Hodgkin lymphoma.

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