RCH-ACV

Cat.No.: CSC-C0556

Species: Human

Source: B cell precursor leukemia

Morphology: small round cells growing singly or in small clumps in suspension; occasional adherent cells present

Culture Properties: suspension

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Cat.No.

CSC-C0556

Description

Established from bone marrow cells taken at relapse of common acute lymphoblastic leukemia (cALL), seven months after diagnosis, from an 8-year-old girl treated with combination chemotherapy

Species

Human

Source

B cell precursor leukemia

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

small round cells growing singly or in small clumps in suspension; occasional adherent cells present

Karyotype

Human flat-moded hyperdiploid karyotype with 2% polyploidy - 43-50<2n>XX, +8, t(1;19)(q23;p13.3) - sideline with idem, +16, +der(19)t(1;19) - carries t(1;19) effecting TCF3-PBX1 (E2A-PBX) fusion - matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 -, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How do you isolate cells from complex biological samples?

The most common cell separation techniques include immunomagnetic cell separation, fluorescence-activated cell sorting (FACS), density gradient centrifugation, immunogenicity cell isolation, and microfluidic cell sorting.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Reliable results

The ability of this company's cell products to remain free of contamination during transport, storage and experimentation is important for obtaining reliable results.

10 May 2023

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