PB-1

Cat.No.: CSC-C2043

Species: Mouse

Source: pre B cells

Morphology: cells are very small and grow mainly in small clusters, but also singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C2043

Description

Established from the bone marrow of a CBA/C57BL mouse in 1995

Species

Mouse

Source

pre B cells

Recommended Medium

85% SuperCult® McCoys 5a Medium + 15% h.i. FBS + 50 ng/ml Human IL-7 + 0.4x MEM vitamins + 1x MEM non-essential amino acids + 0.5x essential amino acids + 1 mM sodium pyruvate + 0.12% sodium bicarbonate + 2 mM L-glutamine + 50 μM mercaptoethanol

Culture Properties

suspension

Morphology

cells are very small and grow mainly in small clusters, but also singly in suspension

Karyotype

Murine near-diploid karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: IL-7 dependency confirmed by 3H-thymidine assay
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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