NUC-5

Cat.No.: CSC-C1385

Species: Mouse

Source: urinary bladder

Morphology: large and flat regular arranged cells, growing in up to 3 cell layers

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Cat.No.

CSC-C1385

Description

Established from an adult female C3H/Law mouse; non-tumorigenic in vitro immortalized urine bladder cell line with aberrant growth factor response, aneuploid (chromosome number 70-75)

Species

Mouse

Source

urinary bladder

Recommended Medium

45% Hams F10 + 45% DMEM + 10% h.i. FBS + 5 μg/ml Human insulin + 5 μg/ml Human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 μM HEPES

Morphology

large and flat regular arranged cells, growing in up to 3 cell layers

Karyotype

Murine hypotetraploid karyotype with 14% polyploidy and 1-3 dmin in 34% cells - 70(64-74)<4n> - 1-3 centric fusion markers present - resembles published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays

Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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