NUC-1

Cat.No.: CSC-C1384

Species: Mouse

Source: urinary bladder

Morphology: spindle cell-like, disorderly arranged cells, growing in multilayers

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Cat.No.

CSC-C1384

Description

Established from an adult female C3H/Law mouse; tumorigenic, invasive in vitro immortalized urine bladder cell line, aneuploid (chromosome number 70-75)

Species

Mouse

Source

urinary bladder

Recommended Medium

45% Hams F10 + 45% DMEM + 10% h.i. FBS + 5 μg/ml Human insulin + 5 μg/ml Human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 μM HEPES

Morphology

spindle cell-like, disorderly arranged cells, growing in multilayers

Karyotype

Hypotetraploid flat-moded murine karyotype with limited centric fusion, dm present in 25% cells - 70(66-75)<4n>

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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