NU-DHL-1

Cat.No.: CSC-C0588

Species: Human

Source: B cell lymphoma

Morphology: single, round to polymorph cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0588

Description

Established from the left inguinal lymph node of a 73-year-old Caucasian man with B-cell Non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma, non-cleaved cell type) in 1982; assigned to GCB-like lymphoma subtype (germinal center B-cell)

Species

Human

Source

B cell lymphoma

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

single, round to polymorph cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 4% polyploidy - 51(46-53)<2n>XY, +5, +8, +9, +12, +12, t(3;8)(p25;q24), i(5p), dup(7)(q21.3q31.1), der(8)t(3;8)(p25;q24), i(9p), del(12)(q11), i(12p), der(14)t(14;18)(q32;q21)x1-3 - sideline with dup(2)(p2?1p2?4) - carrie

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, fm/cykappa -, sm/cylambda +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is a 3D cell culture for cancer?

3D cell culture models replicate the natural tumor architecture, thereby presenting an external proliferating zone, an internal quiescent zone with limited oxygen, nutrient, and growth factor distribution, and a necrotic and hypoxic core. All these factors might influence drug response.

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