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Cat.No.: CSC-C6235X

Species: Human

Source: B lymphoblastoid cells

Morphology: single, polymorph cells or small clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C6235X

Description

Established from peripheral blood lymphocytes of a 27-year-old Caucasian woman by Epstein-Barr virus (EBV) transformation in 1990; suitable normal control cell line for toxicity or radiosensitivity assays

Species

Human

Source

B lymphoblastoid cells

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, polymorph cells or small clumps in suspension

Karyotype

Human near diploid karyotype with hypodiploid sideline - 45/46(42-46)<2n>XX/X, -X - no structural rearrangements detected

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 (+), cyCD79a (+), CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why is ELISA better than Radioimmunoassay?

ELISA detects antibody-antigen complexes using enzymes whereas RIA detects antibody-antigen complexes using radioisotopes. ELISA involves labeling the antibody whereas RIA involves labeling the antigen. ELISA assay is less sensitive when compared to RIA assay.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

High reproducibility

A key factor in reproducibility is to ensure consistent cell conditions, including culture conditions, cell density, and passage numbers. We have obtained definitive experimental results with Creative Bioarray's cell products.

21 June 2022

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