NAMALWA.PNT

Cat.No.: CSC-C0230

Species: Homo sapiens, human

Source: Burkitt lymphoma

Morphology: lymphoblastoid, single cells with irregular forms or small clusters, in suspension

Culture Properties: Suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0230

Description

The NAMALWA.PNT cell line is a subclone of the cell line NAMALWA. Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP.

Species

Homo sapiens, human

Source

Burkitt lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

Suspension

Morphology

lymphoblastoid, single cells with irregular forms or small clusters, in suspension

STR DNA Profile

Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9,3
TPOX: 6,11
vWA: 16

Karyotype

Presence of NAMALWA marker chromosomes confirmed

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD37 (+), HLA-DR +, sm/cyIgM +, sm/cyIgG -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the cell culture techniques?

There are two cell culture techniques to grow cells in culture, monolayers on an artificial substrate (i.e., adherent culture) or free-floating in a culture medium (suspension culture).

Ask a Question