NAMALWA.IPN/45

Cat.No.: CSC-C0232

Species: Human

Source: Burkitt lymphoma

Morphology: lymphoblastoid, single cells with irregular forms or small clusters, in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0232

Description

Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin genes, MYC genes and cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H

Species

Human

Source

Burkitt lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid, single cells with irregular forms or small clusters, in suspension

Karyotype

Human hypodiploid karyotype - 45(42-49)<2n>X, -Y, -3, +7, ins(1;?)(q11;?), ins(3) (q11;q27-29), der(5)t(5;?)(p15;?), der(12)t(12;?)(q24;?), der(15)t(15;?)(p11;?) - NAMALWA marker chromosomes present

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD13 -, CD19 +, CD37 +, HLA-DR +, smIgM +, smIgG -
Viruses: ELISA: reverse transc

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the conditions for cell culture?

Cell cultures should be incubated in an incubator with a tightly regulated temperature and CO2 concentration. Most cell lines grow at 37°C and 5% CO2 with saturating humidity, but some cell types require incubation at lower temperatures and/or lower CO2 concentrations.

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