N3-36

Cat.No.: CSC-C6184X

Species: Mouse

Source: hybridoma (anti-fiber/myosin)

Morphology: single round cells, lightly adherent

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Cat.No.

CSC-C6184X

Description

Described as secreting a mouse monoclonal antibody (IgM) reacting with neonatal and adult fast fibers and myosins in rodent and human on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin

Species

Mouse

Source

hybridoma (anti-fiber/myosin)

Recommended Medium

90% DMEM + 10% h.i.FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids

Morphology

single round cells, lightly adherent

Karyotype

Cytogenetics: murine hypertetraploid karyotype, 20% polyploidy - 78-90 - carries large telocentric and two centric fusion markers

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase positive

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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