MHH-PREB-1

Cat.No.: CSC-C0407

Species: Human

Source: B cell lymphoma

Morphology: round cells growing singly or in clusters in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0407

Description

Established from the lymph node of a 5-year-old Caucasian boy with B cell lymphoblastic non-Hodgkin lymphoma at diagnosis in 1994

Species

Human

Source

B cell lymphoma

Recommended Medium

90-92.5% RPMI-1640 + 7.5-10% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly or in clusters in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 48<2n>XYY, +21, ?ins(2;2)(q21;p15p22), t(8;14)(q24;q32)

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, sm/c

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How accurate is fluorescence in situ hybridization?

The diagnostic accuracy of the FISH assay was comparable to that of microscopy which was 95%.

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