MH-7777A

Cat.No.: CSC-C1096

Species: Rat

Source: hepatoma

Morphology: adherent, polymorph round cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C1096

Description

Established from the liver tumor of a Buffalo rat; tumor cells were propagated in the hind leg

Species

Rat

Source

hepatoma

Recommended Medium

90% DMEM + 10% horse serum. + 10 nM insulin + 10 μM dexamethasone

Culture Properties

monolayer

Morphology

adherent, polymorph round cells growing in monolayers

Karyotype

Near-tetraploid rat karyotype with 17% polyploiy - 73-82<4n>

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative

Storage and Shipping

Frozen with 70% medium, 20% horse serum, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.