MFM-223

Cat.No.: CSC-C0461

Species: Human

Source: breast carcinoma

Morphology: adherent cells growing as monolayer with reticulate growth pattern

Culture Properties: monolayer

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Cat.No.

CSC-C0461

Description

Established from the pleural effusion of a post-menopausal breast cancer patient without any prior treatment (tumor: widespread ductal mammary carcinoma T4 N2 M1 with histological grade III); cells were described to express androgen, estrogen and progesterone receptors

Species

Human

Organism

Human

Source

breast carcinoma

Recommended Medium

MEM + 2mM Glutamine + 10% FBS

Culture Properties

monolayer

Morphology

adherent cells growing as monolayer with reticulate growth pattern

Karyotype

Human highly rearranged near-diploid karyotype with 12% polyploidy - 46(45-46)<2n>X, -X, -2, -5, -6, +7, -9, -10, +11, +12, -14, -15, -16, -17, -18, -19, -22, +10mar, add(5)(p15), der(7)?inv(7)(q1?q2?)dup(7)(q31q35), add(7)(q11;hsr), der(7)add(7)(p22)add(

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why do some scholars believe that there is no such thing as black bug contamination?

Firstly, the small black dots do not affect the appressed growth of the cells. Secondly, small black dots appear in culture only after the cells in poor condition have died. Thirdly, it is normal for particles to move in irregular Brownian motion in liquids when their diameter is small enough.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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13 Mar 2021

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MCF7-382

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with pBact, expression vector under control of the human beta actin promotor, resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6288W INQUIRY

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MCF7-422

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with ER-pBact, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6289W INQUIRY

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MCF7-432

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of the human beta actin promotor resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6290W INQUIRY

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MCF7-488X1

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 5 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6291W INQUIRY

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MCF7-490X1

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 4 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6292W INQUIRY

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MCF7-492X1

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the pZeoSV-LacZ vector, resistant to zeocin, control for MCF7-488X1 and MCF7-490X1.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6293W INQUIRY

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For research use only. Not for any other purpose.