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MCF7/TAMR-7
Cat.No.: CSC-C9506J
Species: Human
Source: Breast
Morphology: Epithelial
Culture Properties: Adherent
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The parental cell line for the MCF7/TAMR-7 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-7 has been established from a clone of cells that survived long term treatment with 1 µM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 µM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line
Never can cryopreserved cells be kept at -20 °C.
Change the culture medium every day and rinse with PBS. The small black spots in the cells may gradually decrease and eventually disappear.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Useful guidance
Thanks to the staff at Creative Bioarray for giving me a lot of guidance.
09 May 2021
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Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with pBact, expression vector under control of the human beta actin promotor, resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion
Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with ER-pBact, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion
Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of the human beta actin promotor resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion
Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 5 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion
Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 4 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion
Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the pZeoSV-LacZ vector, resistant to zeocin, control for MCF7-488X1 and MCF7-490X1.;
Original line: MCF7;
Derived from: pleural effusion
