MC3T3-E1

Cat.No.: CSC-C3037

Species: Mouse

Source: Calvaria

Morphology: Fibroblast-like

Culture Properties: adherent

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Cat.No.

CSC-C3037

Description

Established from the calvaria of an embryo/fetus C57BL/6 mouse. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro. Mineral deposits have been identified as hydroxyapatite. Expression of basic fibroblast growth factor (bFGF) mRNA and protein has been shown to be regulated by treatment with TGF β and bFGF. Prostaglandin F2a has been reported to stimulate DNA synthesis and proliferation by up-regulation of insulin-like growth factor I receptors. MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA.

Species

Mouse

Source

Calvaria

Recommended Medium

SuperCult® AlphaMEM + 2mM Glutamine + 10% h.i. FBS

Culture Properties

adherent

Morphology

Fibroblast-like

Karyotype

Murine hypotetraploid karyotype with 11.5% polyploidy - 72(68-80)<4n> - centric fusion absent

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at ca. 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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