KE-37

Cat.No.: CSC-C0218

Species: Human

Source: T cell leukemia

Morphology: single, round cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0218

Description

Established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979

Species

Human

Source

T cell leukemia

Recommended Medium

90-92.5% RPMI-1640 + 7.5-10% h.i. FBS

Culture Properties

suspension

Morphology

single, round cells in suspension

Karyotype

Human flat-moded hypotetraploid karyotype - 91(86-92)<4n>XXYY, +8, -14, -14, t(7;12)(q32-33;p12-13)x2, der(8)t(8;14)(q24;q11)x4, der(14)t(8;14)(q24;q11)x2 - resembles published karyotype - carries 4 copies of der(8)t(8;14) associated with T-ALL - normal c

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, cyCD3 +, smCD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/b

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the cell cycle GCSE?

The cell cycle is a set of steps that take place in cell division. The cell cycle is how cells duplicate and divide. Cells need to divide all the time to replace dead cells, for growth and for repair.

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