K562/AZQR

Cat.No.: CSC-C9504J

Species: Human

Source: Bone Marrow

Morphology: Lymphoblastoid

Culture Properties: Suspension

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Cat.No.

CSC-C9504J

Description

The drug resistant cell line K562 AZQ has been developed from the parent K562 cell line by treatment with AZQ C215-bis (carboethoxyamino)-3, 6-diazitinyl 1;4 (benzoquinone). The cells have been shown to contain decreased levels of glutathione and superoxoid dismutase. They are cross-resistant to adriamycin, mitzolamide, MMNG and mitmycin. Cells should be challenged with AZQ at every 4th passage at a minimum density of 100,000 cells/ml. It is recommended to cultivate the cells without drug on resuscitation.

Species

Human

Source

Bone Marrow

Recommended Medium

RPMI 1640 + 10% Fetal Bovine Serum (FBS) + 2mM Glutamine. Challenge with 1 mM AZQ every 4th passage.

Culture Properties

Suspension

Morphology

Lymphoblastoid

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the methods of cell collection?

There are 2 main methods commonly used for cell collection, trypsin digestion of cells and scraping of cells with a sterile spatula.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent

Creative Bioarray uses advanced technology to process tumor cell products and I can be sure that I am working with the best products.

24 June 2022

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For research use only. Not for any other purpose.