JVM-2

Cat.No.: CSC-C3434

Species: Human

Source: chronic B cell leukemia

Morphology: lymphoblastoid, round to polygonal cells growing in clusters in suspension

Culture Properties: suspension

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Cat.No.

CSC-C3434

Description

Established from the peripheral blood of a 63-year-old woman with B-prolymphocytic leukemia (B-PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells were described to have immunoglobulin light chain restriction (lambda) and to carry t(11;14) leading to IGH@-BCL1 fusion gene and CCND1 (cyclin D1) activation

Species

Human

Source

chronic B cell leukemia

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid, round to polygonal cells growing in clusters in suspension

Karyotype

Human pseudodiploid karyotype with 1.8% polyploidy - 46(42-46)<2n>XX, t(1;15)(q32.3;q22.1), der(8)t(3;8)(q13;p21), t(11;14)(q13;q32), dup(16)(p11p13.2) - resembles published karyotype - carries t(11;14) associated with CCND1 (cyclin D1) activation

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +
Viruses: ELISA:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the 4 types of smears?

Apart from the conventional method, there are other methods also such as water wash, drop and rest, and blood film methods for preparing smears from blood culture broth.

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