JIMT-1

Cat.No.: CSC-C0592

Species: Human

Source: breast carcinoma

Morphology: epithelial-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0592

Description

Established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described to carry an amplified HER-2 oncogene and to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)

Species

Human

Source

breast carcinoma

Recommended Medium

90% DMEM+ 10% h.i.FBS

Culture Properties

monolayer

Morphology

epithelial-like cells growing in monolayers

Karyotype

Human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56<2n>XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1;6)(p1

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How can I avoid cross-contamination of cells during cell subculture?

When performing culture change or subculture, syringes or droppers should not touch the mouth of the culture flask to avoid bringing cells into the culture flask.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent reproducibility

The quality of Creative Bioarray's products is high and the reproducibility of experiments is excellent.

10 Jan 2022

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For research use only. Not for any other purpose.