HT-93

Cat.No.: CSC-C6232X

Species: Human

Source: acute myeloid leukemia

Morphology: single cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C6232X

Description

Established in 1993 from the peripheral blood of a 66-year-old man with acute promyelocytic leukemia (AML FAB M3) at relapse; described to carry t(15;17) and t(1;12) leading to PML-RARA and ETV6-ARG fusion genes, respectively

Species

Human

Source

acute myeloid leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

single cells growing in suspension

Karyotype

Human near-diploid karyotype; 46(44-46)<2n>XY, t(1;12)(q25;p13), der(2)t(1;2)(q25;q31), der(4)t(1;4)(q21;q25)t(1;12)(q25;p13), der(6)del(6)(p23)t(4;6)(q25;q23), t(15;17)(q22;q21); carries complex t(1;12) and t(15;17) rearrangements effecting ETV6-ABL2 and

Quality Control

Mycoplasma: negative PCR assay
Immunology: CD3 -, CD13 +, CD15 (+), CD19 -, CD33 +, CD34 +, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the laboratory techniques for cell culture?

There are two cell culture techniques to grow cells in culture, as monolayers on an artificial substrate (i.e., adherent culture) or free-floating in culture medium (suspension culture).

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Detailed information

Reputable suppliers of oncology cell products usually provide information about the purity of their products, and Creative Bioarray's cell product information is detailed and comprehensive.

23 June 2022

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