HD-MY-Z

Cat.No.: CSC-C0402

Species: Human

Source: Hodgkin lymphoma

Morphology: variable morphology; semi-adherent, spheroid to fusiform cells growing in clumps in mono- and multilayers, occasional giant cells present

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Cat.No.

CSC-C0402

Description

Established in 1991 from the pleural effusion (rich in Hodgkin/Reed-Sternberg cells) from a 29-year-old patient with nodular sclerosing Hodgkin lymphoma (stage IIIb) refractory to therapy

Species

Human

Source

Hodgkin lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Morphology

variable morphology; semi-adherent, spheroid to fusiform cells growing in clumps in mono- and multilayers, occasional giant cells present

Karyotype

Human highly rearranged hyperdiploid karyotype with 24% polyploidy - 52-55<2n>X/Xdel(X)(p11), +1, +2, +5, +6, +8, +9, -17, -17, -18, +19, +20, +21, +22, +2mar, der(1)t(1;3)(q32;p21), der(2)t(2;?10)(p25;q22), add(3)(q27), del(4)(q22), i(5p), i(5q), der(5)t

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 -, CD10 +, CD13 +, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 -, cyCD68 +, HLA-DR

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the use of immunohistochemistry in tumours?

After the antibodies bind to the antigen in the tissue sample, the enzyme or dye is activated, and the antigen can then be seen under a microscope. Immunohistochemistry is used to help diagnose diseases, such as cancer. It may also be used to help tell the difference between different types of cancer.

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