GEM-218

Description: The ABSa15 cell line is derived from the calcified branchial arches of a gilthead seabream and is capable of extracellular matrix mineralization. Gene expression analysis is suggestive of pre-chondrocyte lineage. Extracellular matrix mineralization occurs on supplementation with calcium chloride, β-glycerophosphate and ascorbic acid. Mineral deposits can be detected as early as two weeks of treatment and increase for up to four weeks. ABSa15 is useful for investigating the mechanism of cell differentiation, extracellular matrix mineralization and also the screening of mineralogenic molecules.

Description: Derived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.

Description: Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

Description: The cells support the growth of grunt fin agent with CPE at 20°C and 25°C. Also susceptible to infectious pancreatic necrosis virus of trout at 28°C.

Description: The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.

Description: CCO was established from the ovaries of a healthy, juvenile channel catfish, Ictalurus punctatus. The cells grow at temperatures from 10°C to 35°C with the optimum at 30°C and with a reported population doubling up to 12 hours. CCO cells have been shown to replicated channel catfish virus but were refractory to 12 other viruses from fish, birds and mammals.