FDCP-1

Cat.No.: CSC-C2761

Species: Mouse

Source: bone marrow

Morphology: single or clustered cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C2761

Description

Established from the long-term bone marrow culture of B6D2F1 mice; cells are IL-3-dependent; their differentiation is blocked; this subclone is responsive to G-CSF (FDCP means "factor dependent cell-Paterson" after the Paterson Insitute, Manchester, UK)

Species

Mouse

Source

bone marrow

Recommended Medium

70% SuperCult® IMDM + 20%horse serum + 10% vol conditioned medium of WEHI-3B cell line

Culture Properties

suspension

Morphology

single or clustered cells growing in suspension

Karyotype

Murine hypodiploid karyotype - 32<2n> - carries 8 centric fusion markers

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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