F-36P

Cat.No.: CSC-C0551

Species: Human

Source: acute myeloid leukemia

Morphology: polymorph cells growing as adherent cells and singly in suspension

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Cat.No.

CSC-C0551

Description

Established at diagnosis from the pleural effusion of a 68-year-old man with acute myeloid leukemia (AML M6) secondary to myelodysplastic syndrome (MDS, subtype refractory anemia with excess of blasts, RAEB) in 1989; cells were described to be proliferatively responsive to GM-CSF and IL-3

Species

Human

Source

acute myeloid leukemia

Recommended Medium

80% RPMI-1640 + 20%h.i. FBS + 10 ng/ml GM-CSF

Morphology

polymorph cells growing as adherent cells and singly in suspension

Karyotype

Human flat-moded hypodiploid karyotype with 6% polyploidy - 41(40-43)<2n>XY, -3, -13, -16, -19, -21, -22, +2mar, ins(X;13)(p11;q?q?), del(3)(p13p24), add(5)(q11), dup(5)(q11q35), der(6)add(6)(p23)dup(6)(q25q27), der(7)add(7)(p13)add(7)(q12), add(9)(q35),

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 -, CD42 -, CD71 +, CD235a +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How do tumors stay alive and grow?

As cancer cells divide, a tumor will develop and grow. Cancer cells have the same needs as normal cells. They need a blood supply to bring oxygen and nutrients to grow and survive. When a tumor is very small, it can easily grow, and it gets oxygen and nutrients from nearby blood vessels.

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