EMT6/CPR

Cat.No.: CSC-C9499J

Species: Mouse

Source: Breast

Morphology: Epithelial

Culture Properties: Adherent

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Cat.No.

CSC-C9499J

Description

The cisplatin-resistant variant of the mouse mammary tumour cell line EMT6/P was derived by continuous in vitro exposure to increasing concentrations of cisplatin over a period of 6 weeks. EMT6/CPR was shown to express 2.5-fold resistance to cisplatin as compared with the sensitive parent line. Development of drug resistance was accompanied by a 1.7-fold increase in cellular glutathione (GSH). Growth for 4 weeks in the absence of cisplatin was reported to lead to loss of resistance phenotype and elevated GSH levels in the resistant cell line EMT6/CPR.

Species

Mouse

Source

Breast

Recommended Medium

MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.25μg/ml, cisplatin + 10% Fetal Bovine Serum (FBS)

Culture Properties

Adherent

Morphology

Epithelial

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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