EM-3

Cat.No.: CSC-C0291

Species: Human

Source: chronic myeloid leukemia in blast crisis

Morphology: single, round to oval cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0291

Description

Established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2

Species

Human

Source

chronic myeloid leukemia in blast crisis

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to oval cells in suspension

Karyotype

Human hypertriploid karyotype - 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5-7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why 5% CO2 for cell culture?

5% CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.

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