EM-2

Cat.No.: CSC-C0292

Species: Human

Source: chronic myeloid leukemia in blast crisis

Morphology: single, round to oval cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0292

Description

Established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3

Species

Human

Source

chronic myeloid leukemia in blast crisis

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to oval cells in suspension

Karyotype

Human hypertriploid karyotype with hypotetraploid sideline - 74(70-86)<3n>X, -X, -X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar - der(5)t(5;?)(q13-15;?), der(9)t(9;22)(q34;q11), i(17q)x2 - in some cells up to three copies of

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does pH affect cell culture?

Changes in pH in the cell culture environment can alter virtually every cellular process like metabolism, cell growth, and membrane potential. Extracellular pH is slightly alkaline and typically 7.3-7.4, while intracellular pH is slightly lower at 7.2.

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