EFM-192A

Cat.No.: CSC-C0358

Species: Human

Source: breast carcinoma

Morphology: epitheloid, adherent growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0358

Description

Established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in molecular aspects; erbB-2 gene is amplified and overexpressed

Species

Human

Source

breast carcinoma

Recommended Medium

80%RPMI-1640 + 20% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid, adherent growing as monolayer

Karyotype

Human flat-moded near-triploid karyotype with 20% polyploidy - 68(60-70)<3n>XXXX, +X, -1, -4, +9, -12, -17, -17, -18, -22, +6mar, add(1)(p22), add(1)(p?32), add(2)(q35/37), add(3)(q27/29), i(15q), add(17)(p12), dminx1 present at 38%

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Where should culture solutions and reagents be placed to avoid physical contamination?

To avoid light the reagents should not be placed in a fridge with a glass door and no isotopes should be placed around the reagents.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent

Creative Bioarray's wide range of cellular products has made my experiments much easier.

14 Sep 2022

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For research use only. Not for any other purpose.