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DU-4475

Cat.No.: CSC-C0464

Species: Human

Source: breast carcinoma

Morphology: large round cells growing singly or in clusters in suspension

Culture Properties: suspension

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Cat.No.
CSC-C0464
Description
Established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developped seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; cells were described to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules and the BCL1 gene
Species
Human
Source
breast carcinoma
Recommended Medium
Culture Properties
suspension
Morphology
large round cells growing singly or in clusters in suspension
Karyotype
Human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93<4n>XXXX, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What causes cross-contamination of cells?

Two or more types of cells are used at the same time and contamination is easily caused by the mixing of apparatus, reagents and others.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Professional

I have always received a satisfactory and professional response when I have asked some questions.

13 Sep 2022


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