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DT-40
Cat.No.: CSC-C1127
Species: Chicken
Source: B cell line
Morphology: round cells growing in clumps of 10 to 100 cells in suspension
Culture Properties: suspension
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Cat.No.
CSC-C1127
Description
Established in 1985 from a bursal lymphoma, which was induced by infection of a 1 day old chicken with Rous-associated virus type 1 (RAV-1), an avian leukosis retrovirus carrying no cell-derived oncogene; cells are described to retain the ability to rearrange the immunoglobulin light chain gene (IgL) and to exhibit a high frequency of homologous gene targeting
Species
Chicken
Source
B cell line
Recommended Medium
80% RPMI 1640 medium supplemented with 4.5g/L glucose, 2 mM L-glutamine + 20% h.i. FBS + 50 μM mercaptoethanol
Culture Properties
suspension
Morphology
round cells growing in clumps of 10 to 100 cells in suspension
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Viruses: SMRV -
Viruses: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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