DOGKIT

Cat.No.: CSC-C0621

Species: Human

Source: Burkitt lymphoma

Morphology: round cells growing singly in suspension

Culture Properties: suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0621

Description

Established from the peripheral blood of a 56-year-old Caucasian man with Burkitt lymphoma after second relapse; described to carry t(8;22) and t(14;18) associated with MYC and BCL2 rearrangements, respectively

Species

Human

Source

Burkitt lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly in suspension

Karyotype

Human flat-moded hyperdiploid karyotype with 3.5% polyploidy; 46-49<2n>XY, +20, dup(1)(q21q42), der(2)t(2;14)(q37;q24)t(14;18)(q32;q21), t(8;22)(q24;q11.2), der(14)t(X;14)(p21;p13)t(14;18)(q32;q21), dup(17)(q11q22), der(18)t(14;18)(q32;q21); supernumerary

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the main difference between flow cytometry and fluorescence-activated cell sorting FACS?

FACS is used as a cell sorter and enriched for a subset of cells which is often then studied in further detail using flow cytometry or other analytical techniques. Flow cytometry is used for cell analysis and is focused on measuring protein expression or co-expression within a mixed population of cells.

Ask a Question