DG-75

Cat.No.: CSC-C0239

Species: Human

Source: Burkitt lymphoma

Morphology: round to polygonal, single or clustered cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0239

Description

Established from the pleural effusion of a 10-year-old boy with Burkitt lymphoma (refractory, terminal) in 1975

Species

Human

Source

Burkitt lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round to polygonal, single or clustered cells in suspension

Karyotype

Human near diploid karyotype with 6% polyploidy - 46<2n>XY, t(8;14)(q24;q32)

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, c

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at 12 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why is FBS added to DMEM media?

Serum is added to the culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins.

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