CTV-1

Cat.No.: CSC-C0216

Species: Human

Source: T cell leukemia

Morphology: round or multiformed cells, singly or clustered in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0216

Description

Allegedly established from the peripheral blood of a 40-year-old man with acute monoblastic leukemia (AML M5) at relapse in 1982; however, recent new data suggest derivation from T-ALL (acute lymphoblastic leukemia): (1) T-ALL-associated gene expression signature (Andersson et al., Leukemia 19: 1042-1050, 2005); (2) own FISH analysis confirming t(1;7) effecting TAL1-TRB@ (TAL1-TRB) fusion; (3) own reanalysis of the immunoprofile

Species

Human

Source

T cell leukemia

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round or multiformed cells, singly or clustered in suspension

Karyotype

Human near tetraploid karyotype with 9% polyploidy - 92(89-94)<4n>XX/XXY/XXYY, -1, +6, +6, -17, t(1;7)(p34.2;q34)x2, del(3)(p21), i(6q)x2, t(12;16)(q24.32;q11)x2

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 (+), CD7 +, CD8 -, CD13 -, CD15 +, CD19 -, CD33 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -
Viruses: ELI

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Is cell viability good?

Alternatively, cell viability can be used to correlate cell behavior to cell number, providing a more accurate picture of cell metabolism. Regardless of the purpose of the experiment, cell viability is extremely important, and there are several methods to assess it accurately.

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