CRO-AP6

Cat.No.: CSC-C0632

Species: Human

Source: B cell lymphoma

Morphology: round to pleomorphic cells growing in small clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0632

Description

Established at diagnosis from the pleural effusion of a 26-year-old Caucasian HIV + man with primary effusion lymphoma (PEL) in 1999; described to be HHV-8 +, EBV - and to display biallelic inactivation of the DNA repair enzyme gene O6-methylguanine-DNA methyltransferase (MGMT) by promoter methylation

Species

Human

Source

B cell lymphoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round to pleomorphic cells growing in small clumps in suspension

Karyotype

Human hyperdiploid karyotype; 48(45-48)<2n>XY, +16, +mar, der(11)t(4;11)(q28;q24), der(13)dup(13)(q1?q3?)t(13;14)(q3?;q21), der(14)t(13;14)(q3?;q21), add(16)(q24)(hsr), i(21)(q10); carries hsr on der(13) and der(16); matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HC

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why do we change cell media?

Medium changes keep the cells healthy by providing fresh nutrients, while cell passage or splitting is required to maintain cells in exponential growth.

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