CRO-AP5

Cat.No.: CSC-C0330

Species: Human

Source: B cell lymphoma

Morphology: single round to pleomorph cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0330

Description

Established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma (PEL) and a history of Kaposis sarcoma; cells are HHV-8-positive, but HIV-negative

Species

Human

Source

B cell lymphoma

Recommended Medium

80-85% RPMI-1640 + 15-20% h.i. FBS

Culture Properties

suspension

Morphology

single round to pleomorph cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -
Viruses: ELISA: reverse trans

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Does DAPI stain both live and dead cells?

DAPI can stain dead cells it prefers to stain dead cells. This has to do with the membrane permeability of dead cells. It cannot stain live cells with intact membranes unless used at high concentrations.

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