CRO-AP2

Cat.No.: CSC-C0220

Species: Human

Source: B cell lymphoma

Morphology: lymphoblast-like cells growing in clumps or as single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0220

Description

Established in 1996 from the diagnostic pre-treatment pleural effusion sampled shortly before death of a 49-year-old HIV+ homosexual man with primary effusion lymphoma (PEL) and previous history of Kaposis sarcoma; autopsy revealed involvement of serous membranes without formation of solid tumor mass; cells were described to be EBV+, HHV-8+ and to carry a BCL6 point mutation

Species

Human

Source

B cell lymphoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblast-like cells growing in clumps or as single cells in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 48(47-48)<2n>X, +X, -Y, +12, add(?Y)(p11), add(1)(q43.1), der(15)t(8;15)(q11;q25), der(22)t(1;22)(q21;q13) - sideline with add(9)(q13) - resembles published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 (+), cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: EL

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What happens during the S phase?

The S phase, occurring between the G1 and G2 phases of the cell cycle, is when DNA replication and much of the DNA repair activity occurs in cells. DNA replication is thought to proceed as large factories, consisting of large clusters of proteins, at the DNA replication fork.

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