COLO-720L

Cat.No.: CSC-C0327

Species: Human

Source: B lymphoblastoid cells (from patient with adenocarcinoma)

Morphology: lymphoblast-like cells growing in suspension as single cells and in clumps

Culture Properties: suspension

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Cat.No.

CSC-C0327

Description

B-lymphoblastoid cell line established from the peripheral blood of a 73-year-old woman with metastatic adenocarcinoma in 1988

Species

Human

Source

B lymphoblastoid cells (from patient with adenocarcinoma)

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblast-like cells growing in suspension as single cells and in clumps

Karyotype

Human hyperdiploid karyotype with 10% polyploidy - 47(45-50)<2n>XX, +12, der(5)t(3;5)(p21.3;p15) - the 3p21.3 breakpoint is associated with benign adenoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, HLA-DR +, sm/cyIgM -, smIg/cyG+, sm/cykappa (+)/+, sm/cylambda -
Viruses: ELISA: reverse transcriptase negative; PCR

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why does DAPI bind to DNA?

It is believed that DAPI associates with the minor groove of double-stranded DNA, with a preference for the adenine-thymine clusters. Cells must be permeabilized and/or fixed for DAPI to enter the cell and bind DNA. Fluorescence increases approximately 20-fold when DAPI is bound to double-stranded DNA.

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