CAL-148

Cat.No.: CSC-C0489

Species: Human

Source: breast adenocarcinoma

Morphology: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0489

Description

Established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)

Species

Human

Source

breast adenocarcinoma

Recommended Medium

80% DMEM + 20% h.i.FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers

Karyotype

Human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originator's unpublished karyotype

Quality Control

Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I do if I find a change in the original cytogenetic material of a cell in culture?

We recommend resuscitating early frozen cells for use and performing genetic identification again.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good brand

Creative Bioarray is a good brand in our minds and their staff are all very responsive.

07 Sep 2021

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For research use only. Not for any other purpose.