BL-70

Cat.No.: CSC-C0339

Species: Human

Source: Burkitt lymphoma

Morphology: single, round cells growing non-adherent in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0339

Description

Established from the tumor tissue at relapse of a 16-year-old Caucasian boy with Burkitt lymphoma; cells were described to be EBV-negative

Species

Human

Source

Burkitt lymphoma

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 μM mercaptoethanol)

Culture Properties

suspension

Morphology

single, round cells growing non-adherent in suspension

Karyotype

Human hyperdiploid karyotype with 4% polyploidy - 47(42-49)<2n>XY, +7, inv(1)(p21q21), del(2)(q33), t(8;14)(q24;q32), t(12;22)(q21.1;q13.2)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transcri

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Where does fetal bovine serum come from?

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter.

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