BL-41

Cat.No.: CSC-C0305

Species: Human

Source: Burkitt lymphoma

Morphology: single, round cells growing non-adherent in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0305

Description

Established from the tumor tissue of an 8-year-old Caucasian boy with Burkitt lymphoma; cells were described to be EBV-negative

Species

Human

Source

Burkitt lymphoma

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 μM mercaptoethanol)

Culture Properties

suspension

Morphology

single, round cells growing non-adherent in suspension

Karyotype

Human hyperdiploid karyotype with 7% polyploidy - 48(42-49)<2n>XY, +7, -13, +2mar, add(8)(q24), t(8;14)(q24;q32), der(15)t(13;15)(q13;p11), add(17)(p12), subclonal rearrangements at 1q23, 7p22, 11q13

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: ELISA: rev

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is DMSO in cell culture?

Dimethyl sulfoxide (DMSO) is an organic compound with the formula of (CH3)2SO. DMSO is frequently used in cell banking applications as a cryoprotectant. DMSO prevents intracellular and extracellular crystals from forming in cells during the freezing process.

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