BE-13

Cat.No.: CSC-C0443

Species: Human

Source: bone marrow

Morphology: single round cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0443

Description

Established from bone marrow cells of an 11-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in relapse; cell lines BE-13 and PEER show identical DNA fingerprints and hence share common origin, presumably due to cross-contamination; however, their diploid and tetraploid karyotypes indicative of earlier and later passage numbers, respectively, suggest that BE-13 is derived from PEER, rather than vice-versa; carries NUP214-ABL1 fusion gene

Species

Human

Source

bone marrow

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

single round cells growing in suspension

Karyotype

Human flat-moded hypotetraploid karyotype - 81-89<4n>XXXX, +1, +4, -9, -9, -10, -16, -21, del(1)(q13.2), add(1)(p11-21), del(4)(q11q31.2)/i(4p)x2, i(4q)del(4q31.2)/i(4q)add(4)(q31)x2, del(5)(q14q21)x2, del(6)(q22)x2, del(9)(p22)x2, der(17)t(17;?21)(p11;q1

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -
Viru

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why is in situ hybridization important?

The objective of in situ hybridization is to determine the presence or absence of DNA or RNA sequences of interest, as well as to localize these sequences to specific cells or chromosomal sites.

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