BD-215

Cat.No.: CSC-C1225

Species: Human

Source: B lymphoblastoid cells (from patient with Fanconi anemia)

Morphology: lymphoblastoid-like cells growing as single cells or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C1225

Description

B lymphoblastoid cell line (B-LCL) established from the peripheral blood of a Scottish man with Fanconi anemia (complementation group C); homozygote with unrelated non-consanguineous parents; homozygous for nonsense mutation in exon 6 of the FANCC (FACC) coding sequences

Species

Human

Source

B lymphoblastoid cells (from patient with Fanconi anemia)

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid-like cells growing as single cells or in clumps in suspension

Karyotype

Human diploid karyotype with 12% polyploidy - 46, XY, 1qh+ - no consistent abnormality detected

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 +, HLA-DR +, sm/cyIgM -, smIgG -, cyIgG +, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse tr

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Is ELISA a cell-based assay?

Cell-based ELISA is a convenient, lysate-free, high throughput, and sensitive assay that can measure the relative amount of protein phosphorylation in cells. The method can also be used for monitoring the effects of various treatments, activators or inhibitors on phosphorylation.

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