BALB/c Mouse Bone Marrow Mesenchymal Stem Cells

CSC-C4348X

Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.
Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.
Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Mouse

Creative Bioarray will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 ?C) until ready for experimental use. Live-cell shipment is also available on request.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1094X