A4-951

Cat.No.: CSC-C6162X

Species: Mouse

Source: hybridoma (anti-fiber/myosin)

Morphology: round cells, lightly adherent, growing singly and in clumps

Culture Properties: suspension

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Cat.No.

CSC-C6162X

Description

Described as secreting a mouse monoclonal antibody (IgG1) reacting with adult slow fibers in rat and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin

Species

Mouse

Source

hybridoma (anti-fiber/myosin)

Recommended Medium

90% DMEM + 10% h.i.FBS + 1x non-essential amino acids

Culture Properties

suspension

Morphology

round cells, lightly adherent, growing singly and in clumps

Karyotype

Murine flat-moded hypertetraploid karyotype - 81(42-92)<4n> - 1-2 dmin and 1-2 centric fusion markers present in most cells

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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